Controllable stripping of radiolabeled group in vivo to optimize nuclear imaging via NO-responsive bioorthogonal cleavage reaction

Evaluation of radiolabeled streptokinase for thrombosis imaging

  Introduction: Cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke. Methods: In this work a recently developed radiolabeled streptokinase (STP) tracer was evaluated in an animal thrombotic model using SPECT imaging and biodis...

RADIOLABELED ANTIBODY TUMOR DOSIMETRY REPORT OF TASK GROUP NO. 2 AAPM NUCLEAR MEDICINE COMMITTEE Members

Site-Specific In Vivo Bioorthogonal Ligation via Chemical Modulation.

A critical limitation of bioorthogonal click chemistry for in vivo applications has been its low reaction efficiency due to the pharmacokinetic barriers, such as blood distribution, circulation, and elimination in living organisms. To identify key factors that dominate the efficiency of click chemistry, here a rational design of near-infrared fluorophores containing tetrazine as a click moiety ...

CRP: Cleavage of Radiolabeled Phosphoproteins

The CRP (Cleavage of Radiolabeled Phosphoproteins) program guides the design and interpretation of experiments to identify protein phosphorylation sites by Edman sequencing of unseparated peptides. Traditionally, phosphorylation sites are determined by cleaving the phosphoprotein and separating the peptides for Edman 32P-phosphate release sequencing. CRP analysis of a phosphoprotein's sequence ...

Preparation and quality control of radiolabeled streptokinase for Thrombosis imaging

Background: In order to diagnose the site of thrombi, radiolabeled streptokinase can be prepared. The radiolabeled compound can be used in imaging of thrombi in many cardiovascular diseases. Materials and Methods: Streptokinase was successively labeled with [67Ga]-gallium chloride using cyclic DTPA-dianhydride. The conjugation with DTPA was optimized for concentration, time and temperature foll...